General Cell Based Assays
ILEX LIFE SCIENCES LLC QuickZyme Human MMP-8 Activity Assay Kit, 96 Assays, 96-well plate format, immunoassay for active or total matrix metalloproteinase 8 (cell culture conditioned medium, serum, plasma, urine, saliva, tissue homogenates samples)
The QuickZyme Human MMP-8 Activity Assay is an enzyme activity immunoassay that enables you to specifically measure both endogenous active MMP-8 and total active MMP-8 (following activation of pro-MMP-8 on the plate with APMA). It can be used for the measurement of MMP-8 activity in various biological samples, such as cell culture conditioned medium, serum, plasma, urine, saliva and tissue homogenates. This 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity. ASSAY RANGE: 10 ¿ 24000 pg/ml. SENSITIVITY: 100 pg/ml (2 h incubation); 24 pg/ml (6 h incubation); 4 pg/ml (24 h incubation). SYNONYMS: Matrix metalloproteinase-8, MMP8, Neutrophil collagenase, PMNL collagenase, PMNL-CL.
Eurofins Discoverx PathHunter eXpress GPR6 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0634E2CP1M)
The PathHunter GPR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR6 and EA-tagged β-Arrestin-2. Activation of GPR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress LGR4 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0516E2ACP1M)
The PathHunter LGR4 (GPR48) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of LGR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged LGR4 and EA-tagged β-Arrestin-2. Activation of LGR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Aat Bioquest Amplite™ Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio. Due to its small size, it rarely causes steric hindrance problem with antibody/antigen complex formation.
Revvity Health Sciences Inc HTRF Mouse Phospho-SLP76 (Ser376) Detection Kit, 50,000 Assay Points
HTRF Mouse Phospho-SLP76 (Ser376) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Phospho-NDRG1 (Thr346) Detection Kit, 50,000 Assay Points
HTRF Phospho-NDRG1 (Thr346) Detection Kit, 50,000 Assay Points
Eurofins Discoverx PathHunter Mouse SSTR2 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse SSTR2 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure SSTR2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human ADRB2 Gs Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human ADRB2 Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure ADRB2 (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human IkB Degradation Cell-Based Assay Kit (THP-1)
The PathHunter Human IkB Degradation Stable Cell Line Assay (THP-1) measures pathway activation-induced degradation of IκB target protein. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human AVPR2 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human AVPR2 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure AVPR2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
ASSAYQUANT TECHNOLOGIES INC Protein 1000 kin assay AQT1232
PhosphoSens-Kinetic Phosphatase Activity Kit 1000 assays - AQT1232
Meso Scale Discovery R-PLEX Human CNTF ASY
The R-PLEX Human CNTF Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures Ciliary Neurotrophic Factor (CNTF) in human serum plasma and cell culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer blocker reagents calibrator and controls The assay has a typical lower limit of detection of 0 08 pg/mL and an upper limit of quantification of 10 000 pg/mL for CNTF
ASSAYQUANT TECHNOLOGIES INC Protein 1000 kin assay AQT1251
PhosphoSens-Kinetic Phosphatase Activity Kit 1000 assays - AQT1251
Eurofins Discoverx PathHunter eXpress F2R (PAR1) CHO-K1 β-Arrestin GPCR Assay, 2pl Kit. (93-0278E2CP0M)
The PathHunter F2R (PAR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. proteases, TFLLRN) based activation of F2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged F2R and EA-tagged β-Arrestin-2. Activation of F2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress A549 G9a Methyltransferase Assay, 2-plate Kit. (96-0003E15CP7M)
The InCELL Hunter eXpress A549 G9a Methyltransferase Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of G9a via detection of G9a levels. This product uses EFC technology. The cell line included overexpresses G9a target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.